Asian Regional Artemia Reference Center, Tianjin University of Science and Technology, China
Postal address:
Asian Regional Artemia Reference Center, Tianjin University of Science and Technology, No. 9th, 13th Avenue, TEDA, Tianjin 300457, China
Contact person:
Liying Sui, suily@tust.edu.cn
Brief description of involvement in Artemia activities or plans:
Asian Regional Artemia Reference Center (AR-ARC) was established in cooperation with Food and Agriculture Organization of the United Nations in 2016, and has been operating since April 2018. AR-ARC affiliates to College of Marine and Environmental Sciences, Tianjin University of Science and Technology. It has fully functional laboratories for aquaculture, nutrition and chemical analysis, molecular biology, microbiology and cell biology study.
AR-ARC focuses on the fundamental research and application of Artemia and other halophilic organisms, enhances the innovative scientific research, and provides services for national and regional marine economy development. AR-ARC promotes and coordinates interdisciplinary and interregional Artemia research, sets up platform for Artemia information exchange, and provides technical consultation and services for Artemia cysts production and its application in larviculture, and facilitate sustainable exploitation and utilization of Artemia resources.
AR-ARC has established Artemia gene bank and database with more than 300 Artemia cyst samples mainly from China and neighbor countries. AR-ARC provides Artemia cyst samples and qualification services for Artemia biometrics, hatching performance and nutritional value analysis, and molecular biology information for scientific study purpose, and larviculture guidance.
AR-ARC currently is carrying out following research activities related to Artemia:
(1) Exploitation and utilization of Artemia resources
Ø Population genetic diversity study, and Artemia germplasm resource evaluation and improvement based on Artemia gene bank;
Ø Key techniques on diapause deactivation of Artemia cysts;
Ø Use of Artemia in marine environmental pollution monitoring and evaluation.
(2) Fundamental research on Artemia
Ø Molecular mechanism of Artemia life phenomenon, physiological process and adaptation；
Ø Molecular mechanism of diapause and sex determination of Artemia.
(3) Effective use of Artemia in larviculture
AR-ARC has setup the experimental platform and system that can be applied in Artemia fundamental research:
Ø In vivo gene function research platform based on microinjection technology
AR-ARC is equipped with the advanced inversion image and micromanipulation system, which provide tools for single cell isolation and collection, and micromanipulation at cellular, embryonic and individual levels, and hence facilitate single cell sequencing and in vivo function study of key genes.
Ø In vitro primary culture of Artemia embryo cell and biosafety early warning platform
AR-ARC has developed the stable primary cell culture system for hemopoietic tissues, hemocytes of crayfish and Artemia embryos, which provide an effective tool for studying the invading pathways and mechanisms of aquatic pathogens into target tissues/cells.
Ø Gnotobiotic Artemia experimental system
AR-ARC is equipped with gnotobiotic Artemia experimental system, with the aim of studying the interaction between microorganisms and aquatic organisms (Artemia), and the prevention of aquatic diseases and its underlying mechanisms.
Weblink: https://www.ararc.tust.edu.cn
Most important Artemia-related papers/books:
Han XK, Ren YZ, Ouyang XM, Zhang B, Sui LY. Construction of a high-density genetic linkage map and QTL mapping for sex and growth traits in Artemia franciscana. Aquaculture, 540:736692. https://doi.org/10.1016/j.aquaculture.2021.736692
Han XK, Zheng YY, Sui LY. 2020. Effect of polystyrene microplastics and temperature on growth, intestinal histology, and immune responses of brine shrimp Artemia franciscana. Journal of Oceanology and Limnology, 1-10. DOI 10.1007/s00343-020-0118-2
Sui LY, Ren BN, Wang SY, Gao MR, Van Stappen G. 2020. Archaea Haloferax supplementation improves Artemia biomass production in hypersaline conditions. Aquaculture, 528:735540. https://doi.org/10.1016/j.aquaculture.2020.735540
Van Stappen G, Sui LY, Hoa NV, Tamtin M, Nyonje B, Renato de Medeiros Rocha Sorgeloos P, Gajardo G. 2019. Review on integrated production of the brine shrimp Artemia in solar salt ponds. Review in Aquaculture, 12: 1054-1071.
Lopes-dos-Santosa RMA., Groota R., Sui LY, Bossier P. and Van Stappen G. 2019. Halophilic bacteria as a food source for the brine shrimp Artemia. Aquaculture, 500:631-639.
Wang SY, Cui XP, Xu RY, Gao MR, Sui LY. 2019. Effect of carbon and nitrogen ratio control on Artemia growth, water quality, biofloc microbial diversity under high salinity and zero-water exchange culture condition. Journal of Oceanology and Limnology, 37(5):1768-1776.
Han XK, Xu RY, Zheng YY, Gao MR, Sui LY. 2019. Development of EST-SSR markers and genetic diversity analysis among three Artemia species from different geographic populations. Crustaceana, 96(7):841-851.
Li RC, Cui XP, Zhang L, Zhang B, Wang XK, Sui LY. 2018. Brine characterization and Artemia population dynamics in Bohai Bay Solar Saltworks, China. Crustaceana, 91(8):1013-1025.